Professor Zhongkang Wang
Prof. Zhongkang Wang
Position:Professor,Dorctoral Supervisor
Address: The School of Life Science, Chongqing University, Chongqing, P. R. of China
EDUCATION
* PhD Biomedical Engineering 2006 Chongqing University，Chongqing；
Dissertation Title：Detection and Quarantine Biotechnology for Plant Pest and Development of Stabilized Diagnostic Reagent Kits
* M.S. Phytopathology, 1984, Southern China Agricultural University, Chongqing, P. R. China.
Thesis title: "Study on the Biological Characteristics of Neovossia horrida."
* B.S. Plant Protection, 1981, Southwest Agricultural University, Chongqing, P. R. China.
PROFESSIONAL EXPERIENCE
Professor from July, 2013 to present, work in the Schooled of Life Science, Chongqing University.
Professor from July, 2001 to June 2013, work in the Colleague of Bioengineering, Chongqing University.
Director of Research Division (1999): in Tropicana China Beihai Food Co. In charge of Integrate Pest Management program, and developed the optimum protocol for Detecting Huanglongbing of Citrus by PCR assay.
Professor from May, 1998 to June 2001, work in Department of Plant Protection and the Center for Biotechnology, SWAU.
Visiting Scientist Oct. 1994 - April, 1997, worked with Dr. J.C. Comstock in Sugarcane Field Station, USDA-ARS, SAA, Canal Point, FL 33438. Program: Detection of Sugarcane Leaf Scald (Xanthomonus albilineans) with isolation, DIA, and Polymerase chain reaction amplification assays. Screen and identification ratoon stunting disease of sugarcane; Detection and diagnosis of sugarcane mosaic virus and yellow leaf syndrome luteovirus with reverse transcription PCR.
Visiting Scientist Sept. 1993 - Oct.1994, worked with Dr. Norman W. Schaad in foreign Disease-Weed Science Research Unit, USDA-ARS, NAA, Frederick, MD 21702. Program: DNA-DNA liquid hybridization for Xanthomonas oryzae and Xanthomonas oryzicola.
Associate Professor 1991-1993; Worked in Department of Plant Protection, SW. Researches focused on the development of Xanthomonas citri rapid detection assays with DIA & ELISA based on monoclonal and polyclonal antibodies.
Assistant Professor, 1985-1990, Worked in Center for Agricultural Biotechnology and Department of Plant Protection, SW. Agricultural University. Study on the etiology and epidemiology of kernel smut of rice caused by Neovossia horrida.
Research interests
Research Description
The research mainly focused on preparation of insect pathogenic fungal pesticides, Infectious mechanism of Entomopathogenic fungi and development process monitoring of microsclerotia, as well as the Detection of plant quarantine microbes, such as CBCD and HLB of citrus. the research works partially turned to the interaction between insect and their pathogens or symbiotic microorganism, the pathogenesis of the insects infected by fungal pathogens.
Courses
Teaching experience
The course for undergraduate students: “Microbiology” .
The course for graduate students and Ph D students: “Advanced Microbiology”.
Academic position
Chinese Plant Protection Society (CPPS)
Chinese Phytopathology Society (CPS)
Chinese Mycology Society (CMS)
Chongqing Microbiology Society(CMS)
Major achievement
Obtained Chinese invention patents total up to 10 items as first inventor
Main Publications(2012-2018)
1. Song ZY, Yin YP, Jiang SS, Liu JJ, Chen H, Wang ZK(*): Comparative transcriptome analysis of microsclerotia development in Nomuraea rileyi. BMC Genomics 2013, 14:411.
2. Jiang SS, Yin YP, Song ZY, Zhou GL, Wang ZK(*): RacA and Cdc42 regulate polarized growth and microsclerotium formation in the dimorphic fungus Nomuraea rileyi. Res Microbiol 2014, 165: 233-242.
3. Liu JJ, Yin YP, Song ZY, Li Y, Jiang SS, Shao CW, Wang ZK(*): NADH:flavin oxidoreductase/NADH oxidase and ROS regulate microsclerotium development in Nomuraea rileyi. World J Microb Biot 2014, 30: 1927-1935.
4. Song ZY, Yin YP, Jiang SS, Liu JJ, Wang ZK(*): Optimization of culture medium for microsclerotia production by Nomuraea rileyi and analysis of their viability for use as a mycoinsecticide. BioControl 2014, 59:597-605.
5. Shao CW, Li R, Yin YP, Qi ZR, Song ZY, Yan L, Wang ZK(*): Agrobacterium tumefaciens mediated transformation of the entomopathogenicity fungus Nomuraea rileyi. Fungal Genet Biol 2015, 83:19-25.
6. Zhou GL, Song ZY, Yin YP, Jiang W, Wang ZK(*): Involvement of alternative oxidase in the regulation of hypha growth and microsclerotia formation in Nomuraea rileyi CQNr01. World J Microb Biot 2015, 31:1343-1352.
7. Song ZY, Zhong Q , Yin YP, Li Y, Shen L, Wang ZK(*): The high osmotic response and cell wall integrity pathways cooperate to regulate morphology, microsclerotia development, and virulence in Metarhizium rileyi. Scientific reports. 2016 6, 38765; doi: 10.1038/srep38765.
8. Song ZY, Shen L, Yin YP, Tan WY, Shao CW, Xu JM, Wang ZK(*): Role of two N. rileyi transmembrane sensors Sho1p and Sln1p in adaptation to stress due to changing culture conditions during microsclerotia development. World J Microb Biot 2015, 31:477-485.
9. Song ZY, Shen L, Yin YP, Tan WY, Shao CW, Xu JM, Wang ZK(*): Role of two Nomuraea rileyi transmembrane sensors Sho1p and Sln1p in adaptation to stress due to changing culture conditions during microsclerotia development. World J Microb Biot 2015, 31:477-485.
10. Li Y, Wang ZK, Liu XE, Song ZY, Li R, Shao CW Yin YP(*): Siderophore biosynthesis but not reductive iron assimilation is essential for the dimorphic fungus Nomuraea rileyi conidiation, dimorphism transition, resistance to oxidative stress, pigmented microsclertium formation, and virulence. Frontier Microbiologia 2016,7:931.
11. Song ZY , Zhong Q , Yin YP, Li Y, Shen L, Wang ZK(*): The high osmotic response and cell wall integrity pathways cooperate to regulate morphology, microsclerotia development, and virulence in Metarhizium rileyi. Scientific Reports 2016
12. Song ZY, Jiang W, Yin YP, Wang ZK(*):: Polarity proteins Mrcdc24 and Mrbem1 required for hypha growth and microsclerotia formation in Metarhizium rileyi. Biocontrol Sci Techn 2016, 26:733-745.
13. Song ZY, Shen L, Zhong Q, Yin YP, Wang ZK(*):Liquid culture production of microsclerotia of Purpureocillium lilacinum for use as bionematicide. Nematology 2016,18(6):719-726.
14. Song ZY, Wang ZK(*): Statistical optimization of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation. Mycology 2017.3
15. Su Y, Wang ZK, Shao CW, Luo YL, Wang L, Yin YP*, An efficient gene disruption method using a positive–negative split-selection marker and Agrobacterium tumefaciens-mediated transformation for Nomuraea rileyi. World Journal of Microbiology and Biotechnology (2018) 34:26.
Awards
AWARDS
* Outstanding Youth Plant pathologist of CPS (1989);
* Received the Science & Technology Advancement Awards (1989, 1990,1992, 1993) issued by Sichuan and Chongqing governments.
“Research on Key Techniques of Insect fungal Pesticides and Development of locust Fungal product” obtained Science and Technology Invitation Awarding for first class in Second contributory 2009, Chongqing Municipal Government.
Postgraduate training
Total up to 20 graduates and 8 Ph.D students graduated and obtained Academic degrees.
The Dissertation Titles of Doctor of Philosophy（2011-2016）
Postal Dr. Song Zhangyong：Research on the Molecular Mechanism of Nomuraea rileyi Microsclerotium Development and Submerged Fermentation Production.
Dr. Chen Huan：Identification and Analysis of Genes Differentially Expressed in Spodoptera litura in Response to N. rileyi .
Dr. Shao Changwen: Screening and Functional Identification of Virulence related Genes of Nomuraea rileyi and Construction of Genetic Transformation System mediated by Agrobacterium tumefaciens.
Dr. Md. Sultan Mahmud: Metarhizium guizhouense strain COMg135 Labeling with GFP and eGFP against peanut white grub ( Coleoptera: Scarabaeoidea).
Dr. Li Yan：The Function Analysis of Iron Uptake and Iron Homeostasis Regulatory-related Genes in Nomuraea rileyi.
Dr. Huang Yi：Explore the Mechanisms of Cantharidin biosynthesis and functional Analysis of related genes in the Blister beetle Mylabris cichorii
Dr. Luo Yuanli：Construction of SSH Library of Brassica Junicea var. tumida Infected by Plasmodiophora brassicae and Identification of Differentially expressed genes
Dr. Chen Yulong：Construction of a genomic Library of Bacillus amyloliquefaciens strain CQBa03 and Study on Mechanism of antimicrobial activity
The Thesis Titles of Master degree （2011-2016）
Shen Jianfei：Identification of high Virulence of Metarhizium Against White Grubs and the Field application Research of new Formulations [D].Chongqing University, 2011
Yu.Haiyong: Defense Function of Spodoptera litura Epidermis Against Nomuraea riley Infection [D].2011.
Zhang Wendi：Zhang Wendi. Cloning and Expression of Gallerimycin from Spodoptera litura Infection [D]. 2011
Zhang Jianwei：Screening, Identification and Cultural Characteristics of a Highly Pathogenic Strain of Metarhizium against Plutella xylostella [D].Chongqing University, 2011
Huang Shan: Induction Culture Microsclerotia of Nomuraea rileyi and Expression Analysis of an Hydrophobin Gene Nrhyd in Nomuraea rileyi [D].Chongqing University, 2012
Li Yan: Identification and Expression Analysis of QM-like Gene and PDI-like from Spodoptera litura after Challenge by Nomuraea rileyi [D].Chongqing University, 2013
Jiang Shasha: RacA and Cdc42 Regulate Polarized Growth and Microsclerotial Formation in the Dimorphic Fungus Nomuraea riley [D]. Chongqing University, 2014
Liu Juanjuan: Functional Analysis of NADH:Flavin oxidoreductase/NADH oxidase and ROS in Nomuraea rileyi Microsclerotia [D].Chongqing University, 2014
Feng Eryan: Gene Cloning, Expression, and Function Analysis of SpL14-3-3ζ in Spodoptera litura in Response to the Entomopathogenic Fungus Nomuraea rileyi [D].Chongqing University, 2014
Liao Buffing: Isolation and Functional Analysis of McMenA, a Gene Encoding a 1,4-dihydroxy-2-naphthoate Octaprenyltransferase in Mylabris cichorii [D]. Chongqing University, 2014
Zha Shenfang: The Gene Cloning and Functional Study of Farnesyl Diphosphate Synthase in Mylabris cichorii [D].Chongqing University, 2015
Wu Xiaofang: Isolation and Identification of the Causal Agent for Kiwifruit Bacterial Canker in Wanzhou District of Chongqing and The Detection Method Construction of Loop-mediated Isothermal Amplification [D].2015
Zhou Guilin: The Gene Cloning and Functional Analysis of an Alternative Oxidase (AOX) in Dimorphic Fungus Nomuraea rileyi CQNr01 [D].Chongqing University, 2015
Jiang Wei:The Gene Cloning and Functional Analysis of Nrcdc42 and Nrbem1 in Dimorphic Fungus Nomuraea rileyi CQNr01 [D].Chongqing University, 2015
Tan Wenyong: Cloning and Functional Analysis of Na+-Ca+ Exchanger and Calcium Channel Inhibitor in N. rileyi 2015
Shen Ling: Functional Analysis of Rho Gene Family in Nomuraea rileyi [D].Chongqing University, 2016
Yuan Lei: Study on Screening of Highly Virulent Fungal Strain Against Delia Antigua and Its Biological Characteristic [D].Chongqing University, 2016
Xia Qingyan: Comparison between Droplet Digital PCR and Quantitative PCR in the Quantitative Detection of Xanthomonas citri subsp.citri [D].Chongqing University, 2016
Contact information
Tel/Fax: (023)-65345572
Mob：13883870620
E-Mail: zkwang@cqu.edu.cn；w-zk@163.com
QQ: 1494899860
