金凯 教授

2001-2008  西南大学  获博士学位
1997-2001  西南大学  获学士学位
2008-至今  重庆大学  历任讲师、副教授、教授
2015-2016  英国阿伯丁大学访问学者

1、分子微生物学 2、昆虫病理学

研究描述
本人的研究兴趣主要有两个方面。一、以病原真菌与寄主昆虫为模式，研究病原菌与寄主之间的相互作用，特别是细胞壁组成和结构变化影响真菌侵染致病的作用机制。真菌细胞壁是真菌细胞外的重要结构，为真菌抵御机械压力和不良环境等提供保护，主要由多糖和蛋白组成。在侵染过程中，真菌细胞壁的结构和组成发生复杂的动态变化，以利于真菌附着寄主体壁、躲避寄主体内免疫识别等。因此，研究侵染致病过程中细胞壁的结构组成变化与真菌侵染致病的关系，对于阐明昆虫病原真菌的侵染致病机理具有重要意义和实际应用价值。二、产孢是真菌无性繁殖的主要方式，与杀虫真菌的生产效率直接相关。丝状真菌通常具有两种产孢模式：正常产孢和微循环产孢。绿僵菌微循环产孢的孢子产量和质量均优于正常产孢，具有良好的应用潜力。营养可调控丝状真菌产孢模式转换，但机制尚不清楚。因此，明确营养调控昆虫病原真菌产孢模式转换的分子机制，有望为提高孢子的生产效率提供理论依据和技术途径。

中国菌物学会终身会员（会员证号：S402202697S）。

发表的论文（*表示通讯作者）：
[1] Li CC, Zhang QP, Xia YX*, Jin K*. MaAreB, a GATA transcription factor, is involved in nitrogen source utilization, stress tolerances and virulence in Metarhizium acridum. Journal of Fungi. 2021, 7(7): 512. https://doi.org/ 10.3390/jof7070512
[2] Wen ZQ, Tian HT, Xia YX*, Jin K*. MaPmt1, a protein O-mannosyltransferase, contributes to virulence through governing the appressorium turgor pressure in Metarhizium acridum. Fungal Genetics and Biology. 2020, 145: 103480. (WOS:000554939500001)
[3] Zhao TT, Wen ZQ, Xia YX*, Jin K*. The transmembrane protein MaSho1 negatively regulates conidial yield by shifting the conidiation pattern in Metarhizium acridum. Applied Microbiology and Biotechnology. 2020, 104(9): 4005–4015. (WOS: 000526375600026)
[4] Zhang MG, Wei QL, Xia YX*, Jin K*. MaPacC, a pH-responsive transcription factor, negatively regulates thermotolerance and contributes to conidiation and virulence in Metarhizium acridum. Current Genetics. 2020, 66(2): 397–408. (WOS:000519454000013)
[5] Zhang JJ, Jiang H, Du YR, Keyhani NO, Xia YX*, Jin K*. Members of chitin synthase family in Metarhizium acridum differentially affect fungal growth, stress tolerances, cell wall integrity and virulence. PLoS Pathogens. 2019, 15(8): e1007964 (WOS:000488322100027)
[6] Zhao TT, Tian HT, Xia YX*, Jin K*. MaPmt4, a protein O-mannosyltransferase, contributes to cell wall integrity, stress tolerance and virulence in Metarhizium acridum. Current Genetics. 2019, 65(4): 1025–1040. (WOS:000475695500023)
[7] Gao PP, Jin K*, Xia YX*. The phosphatase gene MaCdc14 negatively regulates UV-B tolerance by mediating the transcription of melanin synthesis-related genes and contributes to conidiation in Metarhizium acridum. Current Genetics. 2020, 66(1): 141–153. (WOS:000513356600013)
[8] Gao PP, Li MC, Jin K*, Xia YX*. The homeobox gene MaH1 governs microcycle conidiation for increased conidial yield by mediating transcription of conidiation pattern shift-related genes in Metarhizium acridum. Applied Microbiology and Biotechnology. 2019, 103(5): 2251–2262. (WOS:000461392700021)
[9] Zhang J, Wang ZL, Keyhani NO, Peng, GX, Jin K*, Xia YX*. The protein phosphatase gene MaPpt1 acts as a programmer of microcycle conidiation and a negative regulator of UV-B tolerance in Metarhizium acridum. Applied Microbiology and Biotechnology. 2019, 103(3): 1351–1362. (WOS:000459105 000024)
[10] Du YR, Jin K*, Xia YX*. Involvement of MaSom1, a downstream transcriptional factor of cAMP/PKA pathway, in conidial yield, stress tolerances and virulence in Metarhizium acridum. Applied Microbiology and Biotechnology. 2018, 102: 5611–5623. (WOS:000435334900021)
[11] Wei QL, Du YR, Jin K*, and Xia YX*. The Ste12-like transcription factor        MaSte12 is involved in pathogenicity by regulating the appressorium formation in the entomopathogenic fungus, Metarhizium acridum. Applied Microbiology and Biotechnology. 2017, 101:8571–8584. (WOS:000415906900023)
[12] Wang ZL#, Jin K#, Xia YX. Transcriptional analysis of the conidiation pattern                          shift of the entomopathogenic fungus Metarhizium acridum in response to different nutrients. BMC Genomics. 2016, 17: 586. (# equal contribution) (WOS:000381226400011)
[13] Jin K, Peng GX, Liu YC, Xia YX. The acid trehalase, ATM1, contributes to the in    vivo growth and virulence of the entomopathogenic fungus, Metarhizium acridum. Fungal Genetics and Biology. 2015, 77: 61-67. (WOS:000355241300007)
[14] Ma QS#, Jin K#, Peng GX, Xia YX. An ENA ATPase, MaENA1, of Metarhizium acridum influences the Na+-, thermo- and UV-tolerances of conidia and is involved in multiple mechanisms of stress tolerance. Fungal Genetics and Biology. 2015, 83: 68–77. (# equal contribution) (WOS:000362311600008)
[15] Peng GX#, Jin K#, Liu YC, Xia YX. Enhancing the utilization of host trehalose by fungal trehalase improves the virulence of fungal insecticide. Applied Microbiology and Biotechnology. 2015, 99(20):8611–8618. (# equal contribution) (WOS:000361493200025)
[16] Ming Y, Wei QL, Jin K*, and Xia YX*. MaSnf1, a sucrose non-fermenting protein kinase gene, is involved in carbon source utilization, stress tolerance and virulence in Metarhizium acridum. Applied Microbiology and Biotechnology. 2014, 98: 10153–10164. (WOS:000345331700020)
[17] Jin K, Han LR, and Xia YX*. MaMk1, a FUS3/KSS1-type mitogen-activated protein kinase gene, is required for appressorium formation, and insect cuticle penetration of the entomopathogenic fungus Metarhizium acridum. Journal of Invertebrate Pathology. 2014, 115(1): 68–75. (WOS:000329558800011)
[18] Jin K, Ming Y, and Xia YX*. MaHog1, a Hog1-type mitogen-activated protein kinase gene, contributes to stress tolerance and virulence of the entomopathogenic fungus Metarhizium acridum. Microbiology-SGM. 2012, 158(12): 2987–2996. (WOS:000314005300010)
[19] Jin K, Zheng XL, and Xia YX*. Gene expression profiling via multigene concatemers. PLoS One. 2011, 6(1): e15711. (WOS:000286519500025)
[20] Gao Q#, Jin K#, Ying SH#, Zhang YJ#, Xiao GH#, Shang YF, Duan ZB, Hu X, Xie XQ, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang S, Zhong Y, Ma LJ, St Leger RJ, Zhao GP, Pei Y, Feng MG*, Xia Y*, Wang C*. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genetics. 2011, 7(1): e1001264. (# equal contribution) (WOS:000286653500003)
[21] Jin K, Luo ZB, Jiang XD, Zhang YJ, Zhou YH, and Pei Y*. Carbon catabolite repressor gene BbCre1 influences carbon source uptake but does not have a big impact on virulence in Beauveria bassiana. Journal of Invertebrate Pathology. 2011, 106(3): 400–406. (WOS:000287570800009)
[22] Jin K, Zhang YJ, Fang WG, Luo ZB, Zhou YH, and Pei Y*. Carboxylate transporter gene JEN1 from the entomopathogenic fungus Beauveria bassiana is involved in conidiation and virulence. Applied and Environmental Microbiology. 2010, 76(1): 254–263. (WOS:000272992800029)
[23] Jin K, Zhang YJ, Luo ZB, Xiao YH, Fan YH, Wu D, and Pei Y*. An improved method for Beauveria bassiana transformation using phosphinothricin resistance and green fluorescence protein fusion gene bar::egfp as a selectable and visible marker. Biotechnology Letters. 2008, 30(8): 1379–1383. (WOS:000256909200 011)
[24] Yang M, Jin K, and Xia YX*. MaFKS, a β-1,3-glucan synthase, is involved in cell wall integrity, hyperosmotic pressure tolerance and conidiation in Metarhizium acridum. Current Genetics. 2011, 57(4): 253–260. (WOS:00029297 7000004)
[25] Zhang J, Jin K, Xia, YX. Contributions of β-tubulin to cellular morphology, sporulation and virulence in the insect-fungal pathogen, Metarhizium acridum. Fungal Genetics and Biology. 2017, 103:16–24. (WOS:000404089000002)
[26] Cai ZJ, Peng GX, Cao YQ, Liu YC, Jin K and Xia YX*. Trehalose-6-phosphate synthase 1 from Metarhizium anisopliae: clone, expression and properties of the recombinant. Journal of Bioscience and Bioengeering. 2009, 107 (5): 499–505. (WOS:000267506000004)
[27] 金 凯，张永军，罗志兵，林健文，裴炎*. 利用GFP表达系统检测球孢白僵菌侵染昆虫过程. 菌物学报. 2008, 27(3): 377-384.
[28] 夏玉先*，罗义辉，金凯. 高毒力杀虫真菌基因工程菌株选育的进展与方向. 中国生物防治学报. 2011, 27(1): 1-5.

获权的发明专利：
[1]夏玉先、金凯、彭国雄，一种真菌表达载体及其构建和筛选方法，专利号：ZL201110072083.1；2013.5.29获权(C12N 15/80)
[2]夏玉先、金凯、郑小莉，一种多重基因表达的分析方法，专利号：ZL201010232000.6。2012.11.14获权(C12Q 1/68)
[3]金凯、夏玉先、杜彦茹，一种真菌表达载体及其构建方法，专利号：ZL201710725033.6。2021.2.2获权

先后主持国家自然科学基金项目3项，教育部博士点新教师基金1项，重庆市自然基金3项。主研国家“863”重点项目和国家重点研发计划多项。以第一、共同第一和通讯作者先后在PLoS Pathogens、PLoS Genetics、BMC Genomics、Applied and Environmental Microbiology、Applied Microbiology and Biotechnology等杂志发表文章20余篇，获权发明专利3项。

指导的硕士生毕业11人，目前在读硕博研究生7人。
长期招收硕博研究生。欢迎学院本科生加入本实验室从事毕业设计或只是进行课余研究，获取科研经历。

邮箱： jinkai@cqu.edu.cn